VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC

Pawel Mikulski (), Philip Wolff, Tiancong Lu, Mathias Nielsen, Elsa Franco Echevarria, Danling Zhu, Julia I. Questa, Gerhard Saalbach, Carlo Martins and Caroline Dean ()
Additional contact information
Pawel Mikulski: John Innes Centre
Philip Wolff: John Innes Centre
Tiancong Lu: John Innes Centre
Mathias Nielsen: John Innes Centre
Elsa Franco Echevarria: MRC Laboratory of Molecular Biology
Danling Zhu: John Innes Centre
Julia I. Questa: John Innes Centre
Gerhard Saalbach: Biological Chemistry, John Innes Centre
Carlo Martins: Biological Chemistry, John Innes Centre
Caroline Dean: John Innes Centre

Nature Communications, 2022, vol. 13, issue 1, 1-12

Abstract: Abstract Polycomb (PcG) silencing is crucial for development, but how targets are specified remains incompletely understood. The cold-induced Polycomb Repressive Complex 2 (PRC2) silencing of Arabidopsis thaliana FLOWERING LOCUS C (FLC) provides an excellent system to elucidate PcG regulation. Association of the DNA binding protein VAL1 to FLC PcG nucleation regionis an important step. VAL1 co-immunoprecipitates APOPTOSIS AND SPLICING ASSOCIATED PROTEIN (ASAP) complex and PRC1. Here, we show that ASAP and PRC1 are necessary for co-transcriptional repression and chromatin regulation at FLC. ASAP mutants affect FLC transcription in warm conditions, but the rate of FLC silencing in the cold is unaffected. PRC1-mediated H2Aub accumulation increases at the FLC nucleation region during cold, but unlike the PRC2-delivered H3K27me3, does not spread across the locus. H2Aub thus involved in the transition to epigenetic silencing at FLC, facilitating H3K27me3 accumulation and long-term epigenetic memory. Overall, our work highlights the importance of VAL1 as an assembly platform co-ordinating activities necessary for epigenetic silencing at FLC.

Date: 2022
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DOI: 10.1038/s41467-022-32897-7

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