Single cell atlas of spinal cord injury in mice reveals a pro-regenerative signature in spinocerebellar neurons

Kaya J. E. Matson, Daniel E. Russ, Claudia Kathe, Isabelle Hua, Dragan Maric, Yi Ding, Jonathan Krynitsky, Randall Pursley, Anupama Sathyamurthy, Jordan W. Squair, Boaz P. Levi, Gregoire Courtine and Ariel J. Levine ()
Additional contact information
Kaya J. E. Matson: National Institutes of Health
Daniel E. Russ: National Cancer Institute, NIH
Claudia Kathe: École Polytechnique Fédérale de Lausanne (EPFL)
Isabelle Hua: National Institutes of Health
Dragan Maric: National Institute of Neurological Disorders and Stroke
Yi Ding: Allen Institute for Brain Science
Jonathan Krynitsky: National Institutes of Health
Randall Pursley: National Institutes of Health
Anupama Sathyamurthy: National Institutes of Health
Jordan W. Squair: École Polytechnique Fédérale de Lausanne (EPFL)
Boaz P. Levi: Allen Institute for Brain Science
Gregoire Courtine: École Polytechnique Fédérale de Lausanne (EPFL)
Ariel J. Levine: National Institutes of Health

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract After spinal cord injury, tissue distal to the lesion contains undamaged cells that could support or augment recovery. Targeting these cells requires a clearer understanding of their injury responses and capacity for repair. Here, we use single nucleus RNA sequencing to profile how each cell type in the lumbar spinal cord changes after a thoracic injury in mice. We present an atlas of these dynamic responses across dozens of cell types in the acute, subacute, and chronically injured spinal cord. Using this resource, we find rare spinal neurons that express a signature of regeneration in response to injury, including a major population that represent spinocerebellar projection neurons. We characterize these cells anatomically and observed axonal sparing, outgrowth, and remodeling in the spinal cord and cerebellum. Together, this work provides a key resource for studying cellular responses to injury and uncovers the spontaneous plasticity of spinocerebellar neurons, uncovering a potential candidate for targeted therapy.

Date: 2022
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DOI: 10.1038/s41467-022-33184-1

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