Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress

Xi Wang, Fan Zheng, Yuan-yuan Yi, Gao-yuan Wang, Li-xin Hong, Dannel McCollum, Chuanhai Fu (), Yamei Wang () and Quan-wen Jin ()
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Xi Wang: Xiamen University
Fan Zheng: University of Science and Technology of China
Yuan-yuan Yi: Xiamen University
Gao-yuan Wang: Xiamen University
Li-xin Hong: Xiamen University
Dannel McCollum: University of Massachusetts Medical School
Chuanhai Fu: University of Science and Technology of China
Yamei Wang: Xiamen University
Quan-wen Jin: Xiamen University

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Microtubules play a crucial role during the establishment and maintenance of cell polarity. In fission yeast cells, the microtubule plus-end tracking proteins (+TIPs) (including the CLIP-170 homologue Tip1) regulate microtubule dynamics and also transport polarity factors to the cell cortex. Here, we show that the E3 ubiquitin ligase Dma1 plays an unexpected role in controlling polarized growth through ubiquitinating Tip1. Dma1 colocalizes with Tip1 to cortical sites at cell ends, and is required for ubiquitination of Tip1. Although the absence of dma1+ does not cause apparent polar growth defects in vegetatively growing cells, Dma1-mediated Tip1 ubiquitination is required to restrain polar growth upon DNA replication stress. This mechanism is distinct from the previously recognized calcineurin-dependent inhibition of polarized growth. In this work, we establish a link between Dma1-mediated Tip1 ubiquitination and DNA replication or DNA damage checkpoint-dependent inhibition of polarized growth in fission yeast.

Date: 2022
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DOI: 10.1038/s41467-022-33311-y

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