TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy

Chengyang Li, Patrick Duckney, Tong Zhang, Yanshu Fu, Xin Li, Johan Kroon, Geert Jaeger, Yunjiang Cheng, Patrick J. Hussey () and Pengwei Wang ()
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Chengyang Li: Huazhong Agricultural University
Patrick Duckney: Durham University
Tong Zhang: Huazhong Agricultural University
Yanshu Fu: Huazhong Agricultural University
Xin Li: Huazhong Agricultural University
Johan Kroon: Durham University
Geert Jaeger: Ghent University
Yunjiang Cheng: Huazhong Agricultural University
Patrick J. Hussey: Durham University
Pengwei Wang: Huazhong Agricultural University

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract ER-mitochondrial contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising a family of uncharacterised mitochondrial outer membrane proteins, TRB1, TRB2, and the ER protein, VAP27-1. In Arabidopsis, there are three TraB family isoforms and the trb1/trb2 double mutant exhibits abnormal mitochondrial morphology, strong starch accumulation, and impaired energy metabolism, indicating that these proteins are essential for normal mitochondrial function. Moreover, TRB1 and TRB2 proteins also interact with ATG8 in order to regulate mitochondrial degradation (mitophagy). The turnover of depolarised mitochondria is significantly reduced in both trb1/trb2 and VAP27 mutants (vap27-1,3,4,6) under mitochondrial stress conditions, with an increased population of dysfunctional mitochondria present in the cytoplasm. Consequently, plant recovery after stress is significantly perturbed, suggesting that TRB1-regulated mitophagy and ER-mitochondrial interaction are two closely related processes. Taken together, we ascribe a dual role to TraB family proteins which are component of the EMCS complex in eukaryotes, regulating both interaction of the mitochondria to the ER and mitophagy.

Date: 2022
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DOI: 10.1038/s41467-022-33402-w

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