FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli

Zhixin Lyu, Atsushi Yahashiri, Xinxing Yang, Joshua W. McCausland, Gabriela M. Kaus, Ryan McQuillen, David S. Weiss () and Jie Xiao ()
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Zhixin Lyu: Johns Hopkins School of Medicine
Atsushi Yahashiri: University of Iowa Carver College of Medicine
Xinxing Yang: Johns Hopkins School of Medicine
Joshua W. McCausland: Johns Hopkins School of Medicine
Gabriela M. Kaus: University of Iowa Carver College of Medicine
Ryan McQuillen: Johns Hopkins School of Medicine
David S. Weiss: University of Iowa Carver College of Medicine
Jie Xiao: Johns Hopkins School of Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-16

Abstract: Abstract FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate FtsN’s dynamics during sPG synthesis in E. coli. We show that septal FtsN molecules move processively at ~9 nm s−1, the same as FtsWI molecules engaged in sPG synthesis (termed sPG-track), but much slower than the ~30 nm s−1 speed of inactive FtsWI molecules coupled to FtsZ’s treadmilling dynamics (termed FtsZ-track). Importantly, processive movement of FtsN is exclusively coupled to sPG synthesis and is required to maintain active sPG synthesis by FtsWI. Our findings indicate that FtsN is part of the FtsWI sPG synthesis complex, and that while FtsN is often described as a “trigger” for the initiation for cell wall constriction, it must remain part of the processive FtsWI complex to maintain sPG synthesis activity.

Date: 2022
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DOI: 10.1038/s41467-022-33404-8

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