Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

Heewon Cho, Haw-Young Kwon, Amit Sharma, Sun Hyeok Lee, Xiao Liu, Naoki Miyamoto, Jong-Jin Kim, Sin-Hyeog Im, Nam-Young Kang and Young-Tae Chang ()
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Heewon Cho: Pohang University of Science and Technology (POSTECH)
Haw-Young Kwon: Institute for Basic Science (IBS)
Amit Sharma: Pohang University of Science and Technology (POSTECH)
Sun Hyeok Lee: Pohang University of Science and Technology (POSTECH)
Xiao Liu: Pohang University of Science and Technology (POSTECH)
Naoki Miyamoto: Institute for Basic Science (IBS)
Jong-Jin Kim: Sunchon National University
Sin-Hyeog Im: Pohang University of Science and Technology (POSTECH)
Nam-Young Kang: Pohang University of Science and Technology (POSTECH)
Young-Tae Chang: Pohang University of Science and Technology (POSTECH)

Nature Communications, 2022, vol. 13, issue 1, 1-8

Abstract: Abstract Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

Date: 2022
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DOI: 10.1038/s41467-022-33526-z

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