A pathway for chitin oxidation in marine bacteria

Wen-Xin Jiang, Ping-Yi Li (), Xiu-Lan Chen, Yi-Shuo Zhang, Jing-Ping Wang, Yan-Jun Wang, Qi Sheng, Zhong-Zhi Sun, Qi-Long Qin, Xue-Bing Ren, Peng Wang, Xiao-Yan Song, Yin Chen and Yu-Zhong Zhang ()
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Wen-Xin Jiang: Shandong University
Ping-Yi Li: Shandong University
Xiu-Lan Chen: Shandong University
Yi-Shuo Zhang: Shandong University
Jing-Ping Wang: Shandong University
Yan-Jun Wang: Shandong University
Qi Sheng: Shandong University
Zhong-Zhi Sun: Shandong University
Qi-Long Qin: Shandong University
Xue-Bing Ren: Shandong University
Peng Wang: Ocean University of China
Xiao-Yan Song: Shandong University
Yin Chen: Ocean University of China
Yu-Zhong Zhang: Ocean University of China

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract Oxidative degradation of chitin, initiated by lytic polysaccharide monooxygenases (LPMOs), contributes to microbial bioconversion of crystalline chitin, the second most abundant biopolymer in nature. However, our knowledge of oxidative chitin utilization pathways, beyond LPMOs, is very limited. Here, we describe a complete pathway for oxidative chitin degradation and its regulation in a marine bacterium, Pseudoalteromonas prydzensis. The pathway starts with LPMO-mediated extracellular breakdown of chitin into C1-oxidized chitooligosaccharides, which carry a terminal 2-(acetylamino)−2-deoxy-D-gluconic acid (GlcNAc1A). Transmembrane transport of oxidized chitooligosaccharides is followed by their hydrolysis in the periplasm, releasing GlcNAc1A, which is catabolized in the cytoplasm. This pathway differs from the known hydrolytic chitin utilization pathway in enzymes, transporters and regulators. In particular, GlcNAc1A is converted to 2-keto-3-deoxygluconate 6-phosphate, acetate and NH3 via a series of reactions resembling the degradation of D-amino acids rather than other monosaccharides. Furthermore, genomic and metagenomic analyses suggest that the chitin oxidative utilization pathway may be prevalent in marine Gammaproteobacteria.

Date: 2022
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DOI: 10.1038/s41467-022-33566-5

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