Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infection

Taewon Kang, Md Mamunul Haque, Boran Lee, Kyung Tae Hong, Seong Cheol Hong, Younghun Kim, Jesang Lee, Jun-Seok Lee () and Dongwhan Lee ()
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Taewon Kang: Seoul National University, 1 Gwanak-ro, Gwanak-gu
Md Mamunul Haque: Korea University College of Medicine, 73 Goryeodae-ro, Seongbuk-gu
Boran Lee: Seoul National University, 1 Gwanak-ro, Gwanak-gu
Kyung Tae Hong: KIST-School UST, Hwarang-ro 14 gil 5, Seongbuk-gu
Seong Cheol Hong: KIST-School UST, Hwarang-ro 14 gil 5, Seongbuk-gu
Younghun Kim: Seoul National University, 1 Gwanak-ro, Gwanak-gu
Jesang Lee: Seoul National University, 1 Gwanak-ro, Gwanak-gu
Jun-Seok Lee: Korea University College of Medicine, 73 Goryeodae-ro, Seongbuk-gu
Dongwhan Lee: Seoul National University, 1 Gwanak-ro, Gwanak-gu

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract Cell-based assays can monitor virus infection at a single-cell level with high sensitivity and cost-efficiency. For this purpose, it is crucial to develop molecular probes that respond selectively to physiological changes in live cells. We report stimuli-responsive light-emitters built on a T-shaped benzimidazole platform, and consecutive borylation reactions to produce a library of homologs displaying systematic changes in fluorescence quantum yield and environmental sensitivity. We find that certain fluorophores localize selectively at the endoplasmic reticulum, and interact with proteins involved in the stress signaling pathways. Notably, the mono-borylated compound responds selectively to the stress conditions by enhancing fluorescence, and detects avian influenza virus infection at the single-cell level. Our findings demonstrate the unprecedented practical utility of the stress-responsive molecular probes to differentiate cellular states for early diagnosis.

Date: 2022
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DOI: 10.1038/s41467-022-33586-1

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