RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy

Jing Zhang, Bin Wang, Xiaoxiao Gao, Cheng Peng, Chao Shan, Silas F. Johnson, Richard C. Schwartz and Yong-Hui Zheng ()
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Jing Zhang: Chinese Academy of Agricultural Sciences
Bin Wang: Chinese Academy of Agricultural Sciences
Xiaoxiao Gao: Chinese Academy of Sciences
Cheng Peng: Chinese Academy of Sciences
Chao Shan: Chinese Academy of Sciences
Silas F. Johnson: Michigan State University
Richard C. Schwartz: Michigan State University
Yong-Hui Zheng: Chinese Academy of Agricultural Sciences

Nature Communications, 2022, vol. 13, issue 1, 1-18

Abstract: Abstract Virus infection affects cellular proteostasis and provides an opportunity to study this cellular process under perturbation. The proteostasis network in the endoplasmic reticulum (ER) is composed of the calnexin cycle, and the two protein degradation pathways ER-associated protein degradation (ERAD) and ER-to-lysosome-associated degradation (ERLAD/ER-phagy/reticulophagy). Here we show that calnexin and calreticulin trigger Zaire Ebolavirus (EBOV) glycoprotein GP1,2 misfolding. Misfolded EBOV-GP1,2 is targeted by ERAD machinery, but this results in lysosomal instead of proteasomal degradation. Moreover, the ER Ub ligase RNF185, usually associated with ERAD, polyubiquitinates EBOV-GP1,2 on lysine 673 via ubiquitin K27-linkage. Polyubiquinated GP1,2 is subsequently recruited into autophagosomes by the soluble autophagy receptor sequestosome 1 (SQSTM1/p62), in an ATG3- and ATG5-dependent manner. We conclude that EBOV hijacks all three proteostasis mechanisms in the ER to downregulate GP1,2 via polyubiquitination and show that this increases viral fitness. This study identifies linkages among proteostasis network components previously thought to function independently.

Date: 2022
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DOI: 10.1038/s41467-022-33805-9

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