Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors

Hucheng Chang, Neus Gacias Amengual, Alexander Botz, Lorenz Schwaiger, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman and Roland Ludwig ()
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Hucheng Chang: University of Natural Resources and Life Sciences
Neus Gacias Amengual: University of Natural Resources and Life Sciences
Alexander Botz: University of Natural Resources and Life Sciences
Lorenz Schwaiger: University of Natural Resources and Life Sciences
Daniel Kracher: University of Natural Resources and Life Sciences
Stefan Scheiblbrandner: University of Natural Resources and Life Sciences
Florian Csarman: University of Natural Resources and Life Sciences
Roland Ludwig: University of Natural Resources and Life Sciences

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing saccharification efficiency and rate. Recent studies demonstrate that H2O2 rather than O2 is the cosubstrate of the LPMO-catalyzed depolymerization of polysaccharides. Some studies have questioned the physiological relevance of the H2O2-based mechanism for plant cell wall degradation. This study reports the localized and time-resolved determination of LPMO activity on poplar wood cell walls by measuring the H2O2 concentration in their vicinity with a piezo-controlled H2O2 microsensor. The investigated Neurospora crassa LPMO binds to the inner cell wall layer and consumes enzymatically generated H2O2. The results point towards a high catalytic efficiency of LPMO at a low H2O2 concentration that auxiliary oxidoreductases in fungal secretomes can easily generate. Measurements with a glucose microbiosensor additionally demonstrate that LPMO promotes cellobiohydrolase activity on wood cell walls and plays a synergistic role in the fungal extracellular catabolism and in industrial biomass degradation.

Date: 2022
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DOI: 10.1038/s41467-022-33963-w

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