Flexible hyperspectral surface plasmon resonance microscopy

Ziwei Liu, Jingning Wu, Chen Cai, Bo Yang and Zhi-mei Qi ()
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Ziwei Liu: Chinese Academy of Sciences
Jingning Wu: Chinese Academy of Sciences
Chen Cai: Chinese Academy of Sciences
Bo Yang: Chinese Academy of Sciences
Zhi-mei Qi: Chinese Academy of Sciences

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract Optical techniques for visualization and quantification of chemical and biological analytes are always highly desirable. Here we show a hyperspectral surface plasmon resonance microscopy (HSPRM) system that uses a hyperspectral microscope to analyze the selected area of SPR image produced by a prism-based spectral SPR sensor. The HSPRM system enables monochromatic and polychromatic SPR imaging and single-pixel spectral SPR sensing, as well as two-dimensional quantification of thin films with the measured resonance-wavelength images. We performed pixel-by-pixel calibration of the incident angle to remove pixel-to-pixel differences in SPR sensitivity, and demonstrated the HSPRM’s capabilities by using it to quantify monolayer graphene thickness distribution, inhomogeneous protein adsorption and single-cell adhesion. The HSPRM system has a wide spectral range from 400 nm to 1000 nm, an optional field of view from 0.884 mm2 to 0.003 mm2 and a high lateral resolution of 1.2 μm, demonstrating an innovative breakthrough in SPR sensor technology.

Date: 2022
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DOI: 10.1038/s41467-022-34196-7

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