Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Karl-Uwe Reusswig, Julia Bittmann, Martina Peritore, Mathilde Courtes, Benjamin Pardo, Michael Wierer, Matthias Mann and Boris Pfander ()
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Karl-Uwe Reusswig: Max Planck Institute of Biochemistry
Julia Bittmann: Max Planck Institute of Biochemistry
Martina Peritore: Max Planck Institute of Biochemistry
Mathilde Courtes: Université de Montpellier – Centre National de la Recherche Scientifique
Benjamin Pardo: Université de Montpellier – Centre National de la Recherche Scientifique
Michael Wierer: Max Planck Institute of Biochemistry
Matthias Mann: Max Planck Institute of Biochemistry
Boris Pfander: Max Planck Institute of Biochemistry

Nature Communications, 2022, vol. 13, issue 1, 1-20

Abstract: Abstract DNA replicates once per cell cycle. Interfering with the regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineer genetic systems in budding yeast to induce unscheduled replication in a G1-like cell cycle state. Unscheduled G1 replication initiates at canonical S-phase origins. We quantifiy the composition of replisomes in G1- and S-phase and identified firing factors, polymerase α, and histone supply as factors that limit replication outside S-phase. G1 replication per se does not trigger cellular checkpoints. Subsequent replication during S-phase, however, results in over-replication and leads to chromosome breaks and chromosome-wide, strand-biased occurrence of RPA-bound single-stranded DNA, indicating head-to-tail replication collisions as a key mechanism generating genome instability upon G1 replication. Low-level, sporadic induction of G1 replication induces an identical response, indicating findings from synthetic systems are applicable to naturally occurring scenarios of unscheduled replication initiation.

Date: 2022
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DOI: 10.1038/s41467-022-34379-2

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