Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre

Katie J. Harding, Kendra A. Turk-Kubo, Esther Wing Kwan Mak, Peter K. Weber, Xavier Mayali () and Jonathan P. Zehr ()
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Katie J. Harding: University of California
Kendra A. Turk-Kubo: University of California
Esther Wing Kwan Mak: University of California
Peter K. Weber: Lawrence Livermore National Laboratory
Xavier Mayali: Lawrence Livermore National Laboratory
Jonathan P. Zehr: University of California

Nature Communications, 2022, vol. 13, issue 1, 1-10

Abstract: Abstract Biological nitrogen fixation is a major important source of nitrogen for low-nutrient surface oceanic waters. Nitrogen-fixing (diazotrophic) cyanobacteria are believed to be the primary contributors to this process, but the contribution of non-cyanobacterial diazotrophic organisms in oxygenated surface water, while hypothesized to be important, has yet to be demonstrated. In this study, we used simultaneous 15N-dinitrogen and 13C-bicarbonate incubations combined with nanoscale secondary ion mass spectrometry analysis to screen tens of thousands of mostly particle-associated, cell-like regions of interest collected from the North Pacific Subtropical Gyre. These dual isotope incubations allow us to distinguish between non-cyanobacterial and cyanobacterial nitrogen-fixing microorganisms and to measure putative cell-specific nitrogen fixation rates. With this approach, we detect nitrogen fixation by putative non-cyanobacterial diazotrophs in the oxygenated surface ocean, which are associated with organic-rich particles (

Date: 2022
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DOI: 10.1038/s41467-022-34585-y

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