Influenza A virus reassortment in mammals gives rise to genetically distinct within-host subpopulations

Ketaki Ganti, Anish Bagga, Silvia Carnaccini, Lucas M. Ferreri, Ginger Geiger, C. Joaquin Caceres, Brittany Seibert, Yonghai Li, Liping Wang, Taeyong Kwon, Yuhao Li, Igor Morozov, Wenjun Ma, Juergen A. Richt, Daniel R. Perez, Katia Koelle and Anice C. Lowen ()
Additional contact information
Ketaki Ganti: Emory University School of Medicine
Anish Bagga: Emory College of Arts and Sciences
Silvia Carnaccini: University of Georgia
Lucas M. Ferreri: Emory University School of Medicine
Ginger Geiger: University of Georgia
C. Joaquin Caceres: University of Georgia
Brittany Seibert: University of Georgia
Yonghai Li: Kansas State University
Liping Wang: University of Missouri
Taeyong Kwon: Kansas State University
Yuhao Li: Kansas State University
Igor Morozov: Kansas State University
Wenjun Ma: University of Missouri
Juergen A. Richt: Kansas State University
Daniel R. Perez: University of Georgia
Katia Koelle: Emory University
Anice C. Lowen: Emory University School of Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract Influenza A virus (IAV) genetic exchange through reassortment has the potential to accelerate viral evolution and has played a critical role in the generation of multiple pandemic strains. For reassortment to occur, distinct viruses must co-infect the same cell. The spatio-temporal dynamics of viral dissemination within an infected host therefore define opportunity for reassortment. Here, we used wild type and synonymously barcoded variant viruses of a pandemic H1N1 strain to examine the within-host viral dynamics that govern reassortment in guinea pigs, ferrets and swine. The first two species are well-established models of human influenza, while swine are a natural host and a frequent conduit for cross-species transmission and reassortment. Our results show reassortment to be pervasive in all three hosts but less frequent in swine than in ferrets and guinea pigs. In ferrets, tissue-specific differences in the opportunity for reassortment are also evident, with more reassortants detected in the nasal tract than the lower respiratory tract. While temporal trends in viral diversity are limited, spatial patterns are clear, with heterogeneity in the viral genotypes detected at distinct anatomical sites revealing extensive compartmentalization of reassortment and replication. Our data indicate that the dynamics of viral replication in mammals allow diversification through reassortment but that the spatial compartmentalization of variants likely shapes their evolution and onward transmission.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-022-34611-z Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34611-z

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-34611-z

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().