An mTORC1-mediated negative feedback loop constrains amino acid-induced FLCN-Rag activation in renal cells with TSC2 loss

Kaushal Asrani (), Juhyung Woo, Adrianna A. Mendes, Ethan Schaffer, Thiago Vidotto, Clarence Rachel Villanueva, Kewen Feng, Lia Oliveira, Sanjana Murali, Hans B. Liu, Daniela C. Salles, Brandon Lam, Pedram Argani and Tamara L. Lotan ()
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Kaushal Asrani: Johns Hopkins University School of Medicine
Juhyung Woo: Johns Hopkins University School of Medicine
Adrianna A. Mendes: Johns Hopkins University School of Medicine
Ethan Schaffer: Johns Hopkins University School of Medicine
Thiago Vidotto: Johns Hopkins University School of Medicine
Clarence Rachel Villanueva: Johns Hopkins University School of Medicine
Kewen Feng: Johns Hopkins University School of Medicine
Lia Oliveira: Johns Hopkins University School of Medicine
Sanjana Murali: Johns Hopkins University School of Medicine
Hans B. Liu: Johns Hopkins University School of Medicine
Daniela C. Salles: Johns Hopkins University School of Medicine
Brandon Lam: Johns Hopkins University School of Medicine
Pedram Argani: Johns Hopkins University School of Medicine
Tamara L. Lotan: Johns Hopkins University School of Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract The mechanistic target of rapamycin complex 1 (mTORC1) integrates inputs from growth factors and nutrients, but how mTORC1 autoregulates its activity remains unclear. The MiT/TFE transcription factors are phosphorylated and inactivated by mTORC1 following lysosomal recruitment by RagC/D GTPases in response to amino acid stimulation. We find that starvation-induced lysosomal localization of the RagC/D GAP complex, FLCN:FNIP2, is markedly impaired in a mTORC1-sensitive manner in renal cells with TSC2 loss, resulting in unexpected TFEB hypophosphorylation and activation upon feeding. TFEB phosphorylation in TSC2-null renal cells is partially restored by destabilization of the lysosomal folliculin complex (LFC) induced by FLCN mutants and is fully rescued by forced lysosomal localization of the FLCN:FNIP2 dimer. Our data indicate that a negative feedback loop constrains amino acid-induced, FLCN:FNIP2-mediated RagC activity in renal cells with constitutive mTORC1 signaling, and the resulting MiT/TFE hyperactivation may drive oncogenesis with loss of the TSC2 tumor suppressor.

Date: 2022
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DOI: 10.1038/s41467-022-34617-7

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