Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking

Karin Vogel, Tobias Bläske, Marie-Kristin Nagel, Christoph Globisch, Shane Maguire, Lorenz Mattes, Christian Gude, Michael Kovermann, Karin Hauser, Christine Peter and Erika Isono ()
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Karin Vogel: University of Konstanz
Tobias Bläske: University of Konstanz
Marie-Kristin Nagel: University of Konstanz
Christoph Globisch: University of Konstanz
Shane Maguire: University of Konstanz
Lorenz Mattes: University of Konstanz
Christian Gude: Technical University of Munich
Michael Kovermann: University of Konstanz
Karin Hauser: University of Konstanz
Christine Peter: University of Konstanz
Erika Isono: University of Konstanz

Nature Communications, 2022, vol. 13, issue 1, 1-19

Abstract: Abstract The abundance of plasma membrane-resident receptors and transporters has to be tightly regulated by ubiquitin-mediated endosomal degradation for the proper coordination of environmental stimuli and intracellular signaling. Arabidopsis OVARIAN TUMOR PROTEASE (OTU) 11 and OTU12 are plasma membrane-localized deubiquitylating enzymes (DUBs) that bind to phospholipids through a polybasic motif in the OTU domain. Here we show that the DUB activity of OTU11 and OTU12 towards K63-linked ubiquitin is stimulated by binding to lipid membranes containing anionic lipids. In addition, we show that the DUB activity of OTU11 against K6- and K11-linkages is also stimulated by anionic lipids, and that OTU11 and OTU12 can modulate the endosomal degradation of a model cargo and the auxin efflux transporter PIN2-GFP in vivo. Our results suggest that the catalytic activity of OTU11 and OTU12 is tightly connected to their ability to bind membranes and that OTU11 and OTU12 are involved in the fine-tuning of plasma membrane proteins in Arabidopsis.

Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34637-3

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DOI: 10.1038/s41467-022-34637-3

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