Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin

Zhuozhi Chen, Rongdi Duan, Yunjie Xiao, Yi Wei, Hanxiao Zhang, Xinzhao Sun, Shen Wang, Yingying Cheng, Xue Wang, Shanwei Tong, Yunxiao Yao, Cheng Zhu, Haitao Yang, Yanyan Wang () and Zefang Wang ()
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Zhuozhi Chen: Tianjin University
Rongdi Duan: Tianjin University
Yunjie Xiao: Tianjin University
Yi Wei: Tianjin University
Hanxiao Zhang: Tianjin University
Xinzhao Sun: Tianjin University
Shen Wang: Tianjin University
Yingying Cheng: Tianjin University
Xue Wang: Tianjin University
Shanwei Tong: Tianjin University
Yunxiao Yao: Tianjin University
Cheng Zhu: Tianjin University
Haitao Yang: Tianjin University
Yanyan Wang: Tianjin University
Zefang Wang: Tianjin University

Nature Communications, 2022, vol. 13, issue 1, 1-17

Abstract: Abstract The process of recycling poly(ethylene terephthalate) (PET) remains a major challenge due to the enzymatic degradation of high-crystallinity PET (hcPET). Recently, a bacterial PET-degrading enzyme, PETase, was found to have the ability to degrade the hcPET, but with low enzymatic activity. Here we present an engineered whole-cell biocatalyst to simulate both the adsorption and degradation steps in the enzymatic degradation process of PETase to achieve the efficient degradation of hcPET. Our data shows that the adhesive unit hydrophobin and degradation unit PETase are functionally displayed on the surface of yeast cells. The turnover rate of the whole-cell biocatalyst toward hcPET (crystallinity of 45%) dramatically increases approximately 328.8-fold compared with that of purified PETase at 30 °C. In addition, molecular dynamics simulations explain how the enhanced adhesion can promote the enzymatic degradation of PET. This study demonstrates engineering the whole-cell catalyst is an efficient strategy for biodegradation of PET.

Date: 2022
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DOI: 10.1038/s41467-022-34908-z

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