Structures of the eukaryotic ribosome and its translational states in situ

Patrick C. Hoffmann, Jan Philipp Kreysing, Iskander Khusainov, Maarten W. Tuijtel, Sonja Welsch and Martin Beck ()
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Patrick C. Hoffmann: Max Planck Institute of Biophysics
Jan Philipp Kreysing: Max Planck Institute of Biophysics
Iskander Khusainov: Max Planck Institute of Biophysics
Maarten W. Tuijtel: Max Planck Institute of Biophysics
Sonja Welsch: Max Planck Institute of Biophysics
Martin Beck: Max Planck Institute of Biophysics

Nature Communications, 2022, vol. 13, issue 1, 1-9

Abstract: Abstract Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. Different translational states of ribosomes have been well characterized in vitro. However, to which extent the known translational states are representative of the native situation inside cells has thus far only been addressed in prokaryotes. Here, we apply cryo-electron tomography to cryo-FIB milled Dictyostelium discoideum cells combined with subtomogram averaging and classification. We obtain an in situ structure that is locally resolved up to 3 Angstrom, the distribution of eukaryotic ribosome translational states, and unique arrangement of rRNA expansion segments. Our work demonstrates the use of in situ structural biology techniques for identifying distinct ribosome states within the cellular environment.

Date: 2022
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DOI: 10.1038/s41467-022-34997-w

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