Rad52’s DNA annealing activity drives template switching associated with restarted DNA replication

Anastasiya Kishkevich, Sanjeeta Tamang, Michael O. Nguyen, Judith Oehler, Elena Bulmaga, Christos Andreadis, Carl A. Morrow, Manisha Jalan, Fekret Osman and Matthew C. Whitby ()
Additional contact information
Anastasiya Kishkevich: University of Oxford
Sanjeeta Tamang: University of Oxford
Michael O. Nguyen: University of Oxford
Judith Oehler: University of Oxford
Elena Bulmaga: University of Oxford
Christos Andreadis: University of Oxford
Carl A. Morrow: University of Oxford
Manisha Jalan: University of Oxford
Fekret Osman: University of Oxford
Matthew C. Whitby: University of Oxford

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract It is thought that many of the simple and complex genomic rearrangements associated with congenital diseases and cancers stem from mistakes made during the restart of collapsed replication forks by recombination enzymes. It is hypothesised that this recombination-mediated restart process transitions from a relatively accurate initiation phase to a less accurate elongation phase characterised by extensive template switching between homologous, homeologous and microhomologous DNA sequences. Using an experimental system in fission yeast, where fork collapse is triggered by a site-specific replication barrier, we show that ectopic recombination, associated with the initiation of recombination-dependent replication (RDR), is driven mainly by the Rad51 recombinase, whereas template switching, during the elongation phase of RDR, relies more on DNA annealing by Rad52. This finding provides both evidence and a mechanistic basis for the transition hypothesis.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-022-35060-4 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35060-4

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-35060-4

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().