Acousto-holographic reconstruction of whole-cell stiffness maps

Rahmetullah Varol, Zeynep Karavelioglu, Sevde Omeroglu, Gizem Aydemir, Aslihan Karadag, Hanife E. Meco, Ali A. Demircali, Abdurrahim Yilmaz, Gizem C. Kocal, Gulsum Gencoglan, Muhammed E. Oruc, Gokhan B. Esmer, Yasemin Basbinar, Sahin K. Ozdemir and Huseyin Uvet ()
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Rahmetullah Varol: Yildiz Technical University
Zeynep Karavelioglu: Yildiz Technical University
Sevde Omeroglu: Gebze Technical University
Gizem Aydemir: Yildiz Technical University
Aslihan Karadag: Dokuz Eylul University
Hanife E. Meco: Dokuz Eylul University
Ali A. Demircali: Yildiz Technical University
Abdurrahim Yilmaz: Yildiz Technical University
Gizem C. Kocal: Dokuz Eylul University
Gulsum Gencoglan: Istinye University
Muhammed E. Oruc: Gebze Technical University
Gokhan B. Esmer: Marmara University
Yasemin Basbinar: Dokuz Eylul University
Sahin K. Ozdemir: The Pennsylvania State University
Huseyin Uvet: Yildiz Technical University

Nature Communications, 2022, vol. 13, issue 1, 1-10

Abstract: Abstract Accurate assessment of cell stiffness distribution is essential due to the critical role of cell mechanobiology in regulation of vital cellular processes like proliferation, adhesion, migration, and motility. Stiffness provides critical information in understanding onset and progress of various diseases, including metastasis and differentiation of cancer. Atomic force microscopy and optical trapping set the gold standard in stiffness measurements. However, their widespread use has been hampered with long processing times, unreliable contact point determination, physical damage to cells, and unsuitability for multiple cell analysis. Here, we demonstrate a simple, fast, label-free, and high-resolution technique using acoustic stimulation and holographic imaging to reconstruct stiffness maps of single cells. We used this acousto-holographic method to determine stiffness maps of HCT116 and CTC-mimicking HCT116 cells and differentiate between them. Our system would enable widespread use of whole-cell stiffness measurements in clinical and research settings for cancer studies, disease modeling, drug testing, and diagnostics.

Date: 2022
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DOI: 10.1038/s41467-022-35075-x

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