Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light

Kazusato Oikawa, Shino Goto-Yamada, Yasuko Hayashi, Daisuke Takahashi, Yoshitaka Kimori, Michitaro Shibata, Kohki Yoshimoto, Atsushi Takemiya, Maki Kondo, Kazumi Hikino, Akira Kato, Keisuke Shimoda, Haruko Ueda, Matsuo Uemura, Keiji Numata, Yoshinori Ohsumi, Ikuko Hara-Nishimura, Shoji Mano, Kenji Yamada () and Mikio Nishimura ()
Additional contact information
Kazusato Oikawa: National Institute for Basic Biology
Shino Goto-Yamada: Jagiellonian University
Yasuko Hayashi: Niigata University
Daisuke Takahashi: Iwate University
Yoshitaka Kimori: National Institutes of Natural Sciences
Michitaro Shibata: RIKEN Center for Sustainable Resource Science
Kohki Yoshimoto: School of Agriculture
Atsushi Takemiya: Yamaguchi University
Maki Kondo: National Institute for Basic Biology
Kazumi Hikino: National Institute for Basic Biology
Akira Kato: Niigata University
Keisuke Shimoda: Niigata University
Haruko Ueda: Konan University
Matsuo Uemura: Iwate University
Keiji Numata: RIKEN Center for Sustainable Resource Science
Yoshinori Ohsumi: Tokyo Institute of Technology
Ikuko Hara-Nishimura: Konan University
Shoji Mano: National Institute for Basic Biology
Kenji Yamada: Jagiellonian University
Mikio Nishimura: National Institute for Basic Biology

Nature Communications, 2022, vol. 13, issue 1, 1-17

Abstract: Abstract Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.

Date: 2022
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DOI: 10.1038/s41467-022-35138-z

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