Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life

Subhashini Pandey, Manohar Mahato, Preethem Srinath, Utkarsh Bhutani, Tanu Jain Goap, Priusha Ravipati and Praveen Kumar Vemula ()
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Subhashini Pandey: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post
Manohar Mahato: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post
Preethem Srinath: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post
Utkarsh Bhutani: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post
Tanu Jain Goap: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post
Priusha Ravipati: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post
Praveen Kumar Vemula: Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK Post

Nature Communications, 2022, vol. 13, issue 1, 1-15

Abstract: Abstract Transfusion of healthy red blood cells (RBCs) is a lifesaving process. However, upon storing RBCs, a wide range of damage-associate molecular patterns (DAMPs), such as cell-free DNA, nucleosomes, free-hemoglobin, and poly-unsaturated-fatty-acids are generated. DAMPs can further damage RBCs; thus, the quality of stored RBCs declines during the storage and limits their shelf-life. Since these DAMPs consist of either positive or negative charged species, we developed taurine and acridine containing electrospun-nanofibrous-sheets (Tau-AcrNFS), featuring anionic, cationic charges and an DNA intercalating group on their surfaces. We show that Tau-AcrNFS are efficient in scavenging DAMPs from stored human and mice RBCs ex vivo. We find that intermittent scavenging of DAMPs by Tau-AcrNFS during the storage reduces the loss of RBC membrane integrity and reduces discocytes-to-spheroechinocytes transformation in stored-old-RBCs. We perform RBC-transfusion studies in mice to reveal that intermittent removal of DAMPs enhances the quality of stored-old-RBCs equivalent to freshly collected RBCs, and increases their shelf-life by ~22%. Such prophylactic technology may lead to the development of novel blood bags or medical device, and may therefore impact healthcare by reducing transfusion-related adverse effects.

Date: 2022
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DOI: 10.1038/s41467-022-35269-3

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