Chronic exposure to synthetic food colorant Allura Red AC promotes susceptibility to experimental colitis via intestinal serotonin in mice

Yun Han Kwon, Suhrid Banskota, Huaqing Wang, Laura Rossi, Jensine A. Grondin, Saad A. Syed, Yeganeh Yousefi, Jonathan D. Schertzer, Katherine M. Morrison, Michael G. Wade, Alison C. Holloway, Michael G. Surette, Gregory R. Steinberg and Waliul I. Khan ()
Additional contact information
Yun Han Kwon: McMaster University
Suhrid Banskota: McMaster University
Huaqing Wang: McMaster University
Laura Rossi: McMaster University
Jensine A. Grondin: McMaster University
Saad A. Syed: McMaster University
Yeganeh Yousefi: McMaster University
Jonathan D. Schertzer: McMaster University
Katherine M. Morrison: McMaster University
Michael G. Wade: Health Canada
Alison C. Holloway: McMaster University
Michael G. Surette: McMaster University
Gregory R. Steinberg: McMaster University
Waliul I. Khan: McMaster University

Nature Communications, 2022, vol. 13, issue 1, 1-18

Abstract: Abstract Chemicals in food are widely used leading to significant human exposure. Allura Red AC (AR) is a highly common synthetic colorant; however, little is known about its impact on colitis. Here, we show chronic exposure of AR at a dose found in commonly consumed dietary products exacerbates experimental models of colitis in mice. While intermittent exposure is more akin to a typical human exposure, intermittent exposure to AR in mice for 12 weeks, does not influence susceptibility to colitis. However, exposure to AR during early life primes mice to heightened susceptibility to colitis. In addition, chronic exposure to AR induces mild colitis, which is associated with elevated colonic serotonin (5-hydroxytryptamine; 5-HT) levels and impairment of the epithelial barrier function via myosin light chain kinase (MLCK). Importantly, chronic exposure to AR does not influence colitis susceptibility in mice lacking tryptophan hydroxylase 1 (TPH1), the rate limiting enzyme for 5-HT biosynthesis. Cecal transfer of the perturbed gut microbiota by AR exposure worsens colitis severity in the recipient germ-free (GF) mice. Furthermore, chronic AR exposure elevates colonic 5-HT levels in naïve GF mice. Though it remains unknown whether AR has similar effects in humans, our study reveals that chronic long-term exposure to a common synthetic colorant promotes experimental colitis via colonic 5-HT in gut microbiota-dependent and -independent pathway in mice.

Date: 2022
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DOI: 10.1038/s41467-022-35309-y

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