An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing

Feng Xiong, Hong Yang, Yi-Ge Song, Hai-Bin Qin, Qing-Yang Zhang, Xian Huang, Wei Jing, Manfei Deng, Yang Liu, Zhixiang Liu, Yin Shen, Yunyun Han, Youming Lu, Xiangmin Xu, Todd C. Holmes, Minmin Luo, Fei Zhao (), Min-Hua Luo () and Wen-Bo Zeng ()
Additional contact information
Feng Xiong: Chinese Academy of Sciences
Hong Yang: Chinese Academy of Sciences
Yi-Ge Song: Huazhong University of Science and Technology
Hai-Bin Qin: Chinese Academy of Sciences
Qing-Yang Zhang: Chinese Academy of Sciences
Xian Huang: Huazhong University of Science and Technology
Wei Jing: Huazhong University of Science and Technology
Manfei Deng: Huazhong University of Science and Technology
Yang Liu: National Institute of Biological Sciences
Zhixiang Liu: National Institute of Biological Sciences
Yin Shen: Wuhan University
Yunyun Han: Wuhan University
Youming Lu: Huazhong University of Science and Technology
Xiangmin Xu: University of California
Todd C. Holmes: University of California
Minmin Luo: National Institute of Biological Sciences
Fei Zhao: Chinese Institute for Brain Research
Min-Hua Luo: Chinese Academy of Sciences
Wen-Bo Zeng: Chinese Academy of Sciences

Nature Communications, 2022, vol. 13, issue 1, 1-12

Abstract: Abstract Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129Amp tracer system based on HSV-1 strain H129. The H129Amp tracer system consists of two components: an H129-dTK-T2-pacFlox helper which assists H129Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129Amp tracer. These improvements of H129Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.

Date: 2022
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DOI: 10.1038/s41467-022-35355-6

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