Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation

Quanyu Zhou, Zhenyue Chen, Yu-Hang Liu, Mohamad El Amki, Chaim Glück, Jeanne Droux, Michael Reiss, Bruno Weber, Susanne Wegener and Daniel Razansky ()
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Quanyu Zhou: University of Zurich
Zhenyue Chen: University of Zurich
Yu-Hang Liu: University of Zurich
Mohamad El Amki: University Hospital and University of Zurich
Chaim Glück: University of Zurich
Jeanne Droux: University Hospital and University of Zurich
Michael Reiss: University of Zurich
Bruno Weber: University of Zurich
Susanne Wegener: University Hospital and University of Zurich
Daniel Razansky: University of Zurich

Nature Communications, 2022, vol. 13, issue 1, 1-11

Abstract: Abstract Wide-field fluorescence imaging is an indispensable tool for studying large-scale biodynamics. Limited space-bandwidth product and strong light diffusion make conventional implementations incapable of high-resolution mapping of fluorescence biodistribution in three dimensions. We introduce a volumetric wide-field fluorescence microscopy based on optical astigmatism combined with fluorescence source localization, covering 5.6×5.6×0.6 mm3 imaging volume. Two alternative configurations are proposed exploiting multifocal illumination or sparse localization of point emitters, which are herein seamlessly integrated in one system. We demonstrate real-time volumetric mapping of the murine cortical microcirculation at capillary resolution without employing cranial windows, thus simultaneously delivering quantitative perfusion information across both brain hemispheres. Morphological and functional changes of cerebral vascular networks are further investigated after an acute ischemic stroke, enabling cortex-wide observation of concurrent collateral recruitment events occurring on a sub-second scale. The reported technique thus offers a wealth of unmatched possibilities for non- or minimally invasive imaging of biodynamics across scales.

Date: 2022
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DOI: 10.1038/s41467-022-35733-0

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