Oncogenic mutations of PIK3CA lead to increased membrane recruitment driven by reorientation of the ABD, p85 and C-terminus

Meredith L. Jenkins, Harish Ranga-Prasad, Matthew A. H. Parson, Noah J. Harris, Manoj K. Rathinaswamy and John E. Burke ()
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Meredith L. Jenkins: University of Victoria
Harish Ranga-Prasad: University of Victoria
Matthew A. H. Parson: University of Victoria
Noah J. Harris: University of Victoria
Manoj K. Rathinaswamy: University of Victoria
John E. Burke: University of Victoria

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract PIK3CA encoding the phosphoinositide 3-kinase (PI3K) p110α catalytic subunit is frequently mutated in cancer, with mutations occurring widely throughout the primary sequence. The full set of mechanisms underlying how PI3Ks are activated by all oncogenic mutations on membranes are unclear. Using a synergy of biochemical assays and hydrogen deuterium exchange mass spectrometry (HDX-MS), we reveal unique regulatory mechanisms underlying PI3K activation. Engagement of p110α on membranes leads to disengagement of the ABD of p110α from the catalytic core, and the C2 domain from the iSH2 domain of the p85 regulatory subunit. PI3K activation also requires reorientation of the p110α C-terminus, with mutations that alter the inhibited conformation of the C-terminus increasing membrane binding. Mutations at the C-terminus (M1043I/L, H1047R, G1049R, and N1068KLKR) activate p110α through distinct mechanisms, with this having important implications for mutant selective inhibitor development. This work reveals unique mechanisms underlying how PI3K is activated by oncogenic mutations, and explains how double mutants can synergistically increase PI3K activity.

Date: 2023
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DOI: 10.1038/s41467-023-35789-6

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