Uncovering a miltiradiene biosynthetic gene cluster in the Lamiaceae reveals a dynamic evolutionary trajectory

Abigail E. Bryson, Emily R. Lanier, Kin H. Lau, John P. Hamilton, Brieanne Vaillancourt, Davis Mathieu, Alan E. Yocca, Garret P. Miller, Patrick P. Edger, C. Robin Buell and Björn Hamberger ()
Additional contact information
Abigail E. Bryson: Michigan State University
Emily R. Lanier: Michigan State University
Kin H. Lau: Michigan State University
John P. Hamilton: Michigan State University
Brieanne Vaillancourt: Michigan State University
Davis Mathieu: Michigan State University
Alan E. Yocca: Michigan State University
Garret P. Miller: Michigan State University
Patrick P. Edger: Michigan State University
C. Robin Buell: Michigan State University
Björn Hamberger: Michigan State University

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract The spatial organization of genes within plant genomes can drive evolution of specialized metabolic pathways. Terpenoids are important specialized metabolites in plants with diverse adaptive functions that enable environmental interactions. Here, we report the genome assemblies of Prunella vulgaris, Plectranthus barbatus, and Leonotis leonurus. We investigate the origin and subsequent evolution of a diterpenoid biosynthetic gene cluster (BGC) together with other seven species within the Lamiaceae (mint) family. Based on core genes found in the BGCs of all species examined across the Lamiaceae, we predict a simplified version of this cluster evolved in an early Lamiaceae ancestor. The current composition of the extant BGCs highlights the dynamic nature of its evolution. We elucidate the terpene backbones generated by the Callicarpa americana BGC enzymes, including miltiradiene and the terpene (+)-kaurene, and show oxidization activities of BGC cytochrome P450s. Our work reveals the fluid nature of BGC assembly and the importance of genome structure in contributing to the origin of metabolites.

Date: 2023
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DOI: 10.1038/s41467-023-35845-1

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