Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis

Maria Fankhaenel, Farahnaz S. Golestan Hashemi, Larissa Mourao, Emily Lucas, Manal M. Hosawi, Paul Skipp, Xavier Morin, Colinda L.G.J. Scheele and Salah Elias ()
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Maria Fankhaenel: University of Southampton
Farahnaz S. Golestan Hashemi: University of Southampton
Larissa Mourao: VIB-KULeuven Center for Cancer Biology, Herestraat 49
Emily Lucas: University of Southampton
Manal M. Hosawi: University of Southampton
Paul Skipp: University of Southampton
Xavier Morin: PSL Research University
Colinda L.G.J. Scheele: VIB-KULeuven Center for Cancer Biology, Herestraat 49
Salah Elias: University of Southampton

Nature Communications, 2023, vol. 14, issue 1, 1-20

Abstract: Abstract Oriented cell divisions are critical for the formation and maintenance of structured epithelia. Proper mitotic spindle orientation relies on polarised anchoring of force generators to the cell cortex by the evolutionarily conserved protein complex formed by the Gαi subunit of heterotrimeric G proteins, the Leucine-Glycine-Asparagine repeat protein (LGN) and the nuclear mitotic apparatus protein. However, the polarity cues that control cortical patterning of this ternary complex remain largely unknown in mammalian epithelia. Here we identify the membrane-associated protein Annexin A1 (ANXA1) as an interactor of LGN in mammary epithelial cells. Annexin A1 acts independently of Gαi to instruct the accumulation of LGN and nuclear mitotic apparatus protein at the lateral cortex to ensure cortical anchoring of Dynein-Dynactin and astral microtubules and thereby planar alignment of the mitotic spindle. Loss of Annexin A1 randomises mitotic spindle orientation, which in turn disrupts epithelial architecture and luminogenesis in three-dimensional cultures of primary mammary epithelial cells. Our findings establish Annexin A1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.

Date: 2023
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DOI: 10.1038/s41467-023-35881-x

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