Stabilization of membrane topologies by proteinaceous remorin scaffolds

Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V. Mymrikov, Nikolas M. Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra A. M. Fischer, Katharina E. Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse, Winfried Römer and Thomas Ott ()
Additional contact information
Chao Su: University of Freiburg
Marta Rodriguez-Franco: University of Freiburg
Beatrice Lace: University of Freiburg
Nils Nebel: University of Freiburg
Casandra Hernandez-Reyes: University of Freiburg
Pengbo Liang: University of Freiburg
Eija Schulze: University of Freiburg
Evgeny V. Mymrikov: University of Freiburg
Nikolas M. Gross: University of Freiburg
Julian Knerr: University of Freiburg
Hong Wang: University of Freiburg
Lina Siukstaite: University of Freiburg
Jean Keller: Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, INP Toulouse
Cyril Libourel: Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, INP Toulouse
Alexandra A. M. Fischer: University of Freiburg
Katharina E. Gabor: University of Tübingen
Eric Mark: University of Potsdam
Claudia Popp: Ludwig-Maximilians-University (LMU) Munich, Institute of Genetics
Carola Hunte: University of Freiburg
Wilfried Weber: University of Freiburg
Petra Wendler: University of Potsdam
Thomas Stanislas: University of Tübingen
Pierre-Marc Delaux: Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, INP Toulouse
Oliver Einsle: University of Freiburg
Robert Grosse: University of Freiburg
Winfried Römer: University of Freiburg
Thomas Ott: University of Freiburg

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract In plants, the topological organization of membranes has mainly been attributed to the cell wall and the cytoskeleton. Additionally, few proteins, such as plant-specific remorins have been shown to function as protein and lipid organizers. Root nodule symbiosis requires continuous membrane re-arrangements, with bacteria being finally released from infection threads into membrane-confined symbiosomes. We found that mutations in the symbiosis-specific SYMREM1 gene result in highly disorganized perimicrobial membranes. AlphaFold modelling and biochemical analyses reveal that SYMREM1 oligomerizes into antiparallel dimers and may form a higher-order membrane scaffolding structure. This was experimentally confirmed when expressing this and other remorins in wall-less protoplasts is sufficient where they significantly alter and stabilize de novo membrane topologies ranging from membrane blebs to long membrane tubes with a central actin filament. Reciprocally, mechanically induced membrane indentations were equally stabilized by SYMREM1. Taken together we describe a plant-specific mechanism that allows the stabilization of large-scale membrane conformations independent of the cell wall.

Date: 2023
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DOI: 10.1038/s41467-023-35976-5

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