Mechanism of regulation of the Helicobacter pylori Cagβ ATPase by CagZ

Xiuling Wu, Yanhe Zhao, Hong Zhang, Wendi Yang, Jinbo Yang, Lifang Sun, Meiqin Jiang, Qin Wang, Qianchao Wang, Xianren Ye, Xuewu Zhang () and Yunkun Wu ()
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Xiuling Wu: Fujian Normal University
Yanhe Zhao: University of Texas Southwestern Medical Center
Hong Zhang: Fujian Normal University
Wendi Yang: Fujian Normal University
Jinbo Yang: Fujian Normal University
Lifang Sun: Fujian Normal University
Meiqin Jiang: Fujian Normal University
Qin Wang: Binzhou Medical University
Qianchao Wang: Fujian Normal University
Xianren Ye: Fujian Cancer Hospital & Fujian Medical University Cancer Hospital
Xuewu Zhang: University of Texas Southwestern Medical Center
Yunkun Wu: Fujian Normal University

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract The transport of the CagA effector into gastric epithelial cells by the Cag Type IV secretion system (Cag T4SS) of Helicobacter pylori (H. pylori) is critical for pathogenesis. CagA is recruited to Cag T4SS by the Cagβ ATPase. CagZ, a unique protein in H. pylori, regulates Cagβ-mediated CagA transport, but the underlying mechanisms remain unclear. Here we report the crystal structure of the cytosolic region of Cagβ, showing a typical ring-like hexameric assembly. The central channel of the ring is narrow, suggesting that CagA must unfold for transport through the channel. Our structure of CagZ in complex with the all-alpha domain (AAD) of Cagβ shows that CagZ adopts an overall U-shape and tightly embraces Cagβ. This binding mode of CagZ is incompatible with the formation of the Cagβ hexamer essential for the ATPase activity. CagZ therefore inhibits Cagβ by trapping it in the monomeric state. Based on these findings, we propose a refined model for the transport of CagA by Cagβ.

Date: 2023
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DOI: 10.1038/s41467-023-36218-4

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