An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

Sverre Grødem, Ingeborg Nymoen, Guro Helén Vatne, Frederik Sebastian Rogge, Valgerður Björnsdóttir, Kristian Kinden Lensjø () and Marianne Fyhn
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Sverre Grødem: University of Oslo
Ingeborg Nymoen: University of Oslo
Guro Helén Vatne: University of Oslo
Frederik Sebastian Rogge: University of Oslo
Valgerður Björnsdóttir: University of Oslo
Kristian Kinden Lensjø: University of Oslo
Marianne Fyhn: University of Oslo

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Genetically encoded Ca2+ indicators (GECIs) are widely used to measure neural activity. Here, we explore the use of systemically administered PHP.eB AAVs for brain-wide expression of GECIs and compare the expression properties to intracerebrally injected AAVs in male mice. We show that systemic administration is a promising strategy for imaging neural activity. Next, we establish the use of EE-RR- (soma) and RPL10a (Ribo) soma-targeting peptides with the latest jGCaMP and show that EE-RR-tagged jGCaMP8 gives rise to strong expression but limited soma-targeting. In contrast, Ribo-tagged jGCaMP8 lacks neuropil signal, but the expression rate is reduced. To combat this, we modified the linker region of the Ribo-tag (RiboL1-). RiboL1-jGCaMP8 expresses faster than Ribo-jGCaMP8 but remains too dim for reliable use with systemic virus administration. However, intracerebral injections of the RiboL1-tagged jGCaMP8 constructs provide strong Ca2+ signals devoid of neuropil contamination, with remarkable labeling density.

Date: 2023
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DOI: 10.1038/s41467-023-36324-3

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