Engraftment of allogeneic iPS cell-derived cartilage organoid in a primate model of articular cartilage defect

Kengo Abe, Akihiro Yamashita, Miho Morioka, Nanao Horike, Yoshiaki Takei, Saeko Koyamatsu, Keisuke Okita, Shuichi Matsuda and Noriyuki Tsumaki ()
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Kengo Abe: Osaka University
Akihiro Yamashita: Osaka University
Miho Morioka: Osaka University
Nanao Horike: Osaka University
Yoshiaki Takei: Kyoto University
Saeko Koyamatsu: Osaka University
Keisuke Okita: Kyoto University
Shuichi Matsuda: Kyoto University
Noriyuki Tsumaki: Osaka University

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Induced pluripotent stem cells (iPSCs) are a promising resource for allogeneic cartilage transplantation to treat articular cartilage defects that do not heal spontaneously and often progress to debilitating conditions, such as osteoarthritis. However, to the best of our knowledge, allogeneic cartilage transplantation into primate models has never been assessed. Here, we show that allogeneic iPSC-derived cartilage organoids survive and integrate as well as are remodeled as articular cartilage in a primate model of chondral defects in the knee joints. Histological analysis revealed that allogeneic iPSC-derived cartilage organoids in chondral defects elicited no immune reaction and directly contributed to tissue repair for at least four months. iPSC-derived cartilage organoids integrated with the host native articular cartilage and prevented degeneration of the surrounding cartilage. Single-cell RNA-sequence analysis indicated that iPSC-derived cartilage organoids differentiated after transplantation, acquiring expression of PRG4 crucial for joint lubrication. Pathway analysis suggested the involvement of SIK3 inactivation. Our study outcomes suggest that allogeneic transplantation of iPSC-derived cartilage organoids may be clinically applicable for the treatment of patients with chondral defects of the articular cartilage; however further assessment of functional recovery long term after load bearing injuries is required.

Date: 2023
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DOI: 10.1038/s41467-023-36408-0

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