Lipopolysaccharide biosynthesis and traffic in the envelope of the pathogen Brucella abortus

Caroline Servais, Victoria Vassen, Audrey Verhaeghe, Nina Küster, Elodie Carlier, Léa Phégnon, Aurélie Mayard, Nicolas Auberger, Stéphane Vincent and Xavier De Bolle ()
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Caroline Servais: Narilis, University of Namur (UNamur)
Victoria Vassen: Narilis, University of Namur (UNamur)
Audrey Verhaeghe: Narilis, University of Namur (UNamur)
Nina Küster: Narilis, University of Namur (UNamur)
Elodie Carlier: Narilis, University of Namur (UNamur)
Léa Phégnon: Narilis, University of Namur (UNamur)
Aurélie Mayard: Narilis, University of Namur (UNamur)
Nicolas Auberger: Université de Poitiers, IC2MP, UMR CNRS 7285, Equipe “OrgaSynth”, Groupe Glycochimie
Stéphane Vincent: Narilis, University of Namur (UNamur)
Xavier De Bolle: Narilis, University of Namur (UNamur)

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Lipopolysaccharide is essential for most Gram-negative bacteria as it is a main component of the outer membrane. In the pathogen Brucella abortus, smooth lipopolysaccharide containing the O-antigen is required for virulence. Being part of the Rhizobiales, Brucella spp. display unipolar growth and lipopolysaccharide was shown to be incorporated at the active growth sites, i.e. the new pole and the division site. By localizing proteins involved in the lipopolysaccharide transport across the cell envelope, from the inner to the outer membrane, we show that the lipopolysaccharide incorporation sites are determined by the inner membrane complex of the lipopolysaccharide transport system. Moreover, we identify the main O-antigen ligase of Brucella spp. involved in smooth lipopolysaccharide synthesis. Altogether, our data highlight a layer of spatiotemporal organization of the lipopolysaccharide biosynthesis pathway and identify an original class of bifunctional O-antigen ligases.

Date: 2023
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DOI: 10.1038/s41467-023-36442-y

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