Rhodobacter capsulatus forms a compact crescent-shaped LH1–RC photocomplex

Kazutoshi Tani (), Ryo Kanno, Xuan-Cheng Ji, Itsusei Satoh, Yuki Kobayashi, Malgorzata Hall, Long-Jiang Yu, Yukihiro Kimura, Akira Mizoguchi, Bruno M. Humbel, Michael T. Madigan and Zheng-Yu Wang-Otomo ()
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Kazutoshi Tani: Mie University
Ryo Kanno: Okinawa Institute of Science and Technology Graduate University (OIST)
Xuan-Cheng Ji: Ibaraki University
Itsusei Satoh: Ibaraki University
Yuki Kobayashi: Ibaraki University
Malgorzata Hall: Okinawa Institute of Science and Technology Graduate University (OIST)
Long-Jiang Yu: Chinese Academy of Sciences
Yukihiro Kimura: Kobe University
Akira Mizoguchi: Mie University
Bruno M. Humbel: Okinawa Institute of Science and Technology Graduate University (OIST)
Michael T. Madigan: Southern Illinois University
Zheng-Yu Wang-Otomo: Ibaraki University

Nature Communications, 2023, vol. 14, issue 1, 1-9

Abstract: Abstract Rhodobacter (Rba.) capsulatus has been a favored model for studies of all aspects of bacterial photosynthesis. This purple phototroph contains PufX, a polypeptide crucial for dimerization of the light-harvesting 1–reaction center (LH1–RC) complex, but lacks protein-U, a U-shaped polypeptide in the LH1–RC of its close relative Rba. sphaeroides. Here we present a cryo-EM structure of the Rba. capsulatus LH1–RC purified by DEAE chromatography. The crescent-shaped LH1–RC exhibits a compact structure containing only 10 LH1 αβ-subunits. Four αβ-subunits corresponding to those adjacent to protein-U in Rba. sphaeroides were absent. PufX in Rba. capsulatus exhibits a unique conformation in its N-terminus that self-associates with amino acids in its own transmembrane domain and interacts with nearby polypeptides, preventing it from interacting with proteins in other complexes and forming dimeric structures. These features are discussed in relation to the minimal requirements for the formation of LH1–RC monomers and dimers, the spectroscopic behavior of both the LH1 and RC, and the bioenergetics of energy transfer from LH1 to the RC.

Date: 2023
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DOI: 10.1038/s41467-023-36460-w

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