Membrane-localized expression, production and assembly of Vibrio parahaemolyticus T3SS2 provides evidence for transertion

Karan Gautam Kaval, Suneeta Chimalapati, Sara D. Siegel, Nalleli Garcia, Jananee Jaishankar, Ankur B. Dalia and Kim Orth ()
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Karan Gautam Kaval: University of Texas Southwestern Medical Center
Suneeta Chimalapati: Reata Pharmaceuticals
Sara D. Siegel: North Carolina State University
Nalleli Garcia: University of Florida
Jananee Jaishankar: University of Texas Southwestern Medical Center
Ankur B. Dalia: Indiana University
Kim Orth: University of Texas Southwestern Medical Center

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract It has been proposed that bacterial membrane proteins may be synthesized and inserted into the membrane by a process known as transertion, which involves membrane association of their encoding genes, followed by coupled transcription, translation and membrane insertion. Here, we provide evidence supporting that the pathogen Vibrio parahaemolyticus uses transertion to assemble its type III secretion system (T3SS2), to inject virulence factors into host cells. We propose a two-step transertion process where the membrane-bound co-component receptor (VtrA/VtrC) is first activated by bile acids, leading to membrane association and expression of its target gene, vtrB, located in the T3SS2 pathogenicity island. VtrB, the transmembrane transcriptional activator of T3SS2, then induces the localized expression and membrane assembly of the T3SS2 structural components and its effectors. We hypothesize that the proposed transertion process may be used by other enteric bacteria for efficient assembly of membrane-bound molecular complexes in response to extracellular signals.

Date: 2023
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DOI: 10.1038/s41467-023-36762-z

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