TRAK adaptors regulate the recruitment and activation of dynein and kinesin in mitochondrial transport

John T. Canty (), Andrew Hensley, Merve Aslan, Amanda Jack and Ahmet Yildiz ()
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John T. Canty: University of California at Berkeley
Andrew Hensley: University of California at Berkeley
Merve Aslan: University of California at Berkeley
Amanda Jack: University of California at Berkeley
Ahmet Yildiz: University of California at Berkeley

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract Mitochondrial transport along microtubules is mediated by Miro1 and TRAK adaptors that recruit kinesin-1 and dynein-dynactin. To understand how these opposing motors are regulated during mitochondrial transport, we reconstitute the bidirectional transport of Miro1/TRAK along microtubules in vitro. We show that the coiled-coil domain of TRAK activates dynein-dynactin and enhances the motility of kinesin-1 activated by its cofactor MAP7. We find that TRAK adaptors that recruit both motors move towards kinesin-1’s direction, whereas kinesin-1 is excluded from binding TRAK transported by dynein-dynactin, avoiding motor tug-of-war. We also test the predictions of the models that explain how mitochondrial transport stalls in regions with elevated Ca2+. Transport of Miro1/TRAK by kinesin-1 is not affected by Ca2+. Instead, we demonstrate that the microtubule docking protein syntaphilin induces resistive forces that stall kinesin-1 and dynein-driven motility. Our results suggest that mitochondrial transport stalls by Ca2+-mediated recruitment of syntaphilin to the mitochondrial membrane, not by disruption of the transport machinery.

Date: 2023
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DOI: 10.1038/s41467-023-36945-8

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