Multiplex translaminar imaging in the spinal cord of behaving mice

Pavel Shekhtmeyster, Erin M. Carey, Daniela Duarte, Alexander Ngo, Grace Gao, Nicholas A. Nelson, Charles L. Clark and Axel Nimmerjahn ()
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Pavel Shekhtmeyster: The Salk Institute for Biological Studies
Erin M. Carey: The Salk Institute for Biological Studies
Daniela Duarte: The Salk Institute for Biological Studies
Alexander Ngo: The Salk Institute for Biological Studies
Grace Gao: The Salk Institute for Biological Studies
Nicholas A. Nelson: The Salk Institute for Biological Studies
Charles L. Clark: The Salk Institute for Biological Studies
Axel Nimmerjahn: The Salk Institute for Biological Studies

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract While the spinal cord is known to play critical roles in sensorimotor processing, including pain-related signaling, corresponding activity patterns in genetically defined cell types across spinal laminae have remained challenging to investigate. Calcium imaging has enabled cellular activity measurements in behaving rodents but is currently limited to superficial regions. Here, using chronically implanted microprisms, we imaged sensory and motor-evoked activity in regions and at speeds inaccessible by other high-resolution imaging techniques. To enable translaminar imaging in freely behaving animals through implanted microprisms, we additionally developed wearable microscopes with custom-compound microlenses. This system addresses multiple challenges of previous wearable microscopes, including their limited working distance, resolution, contrast, and achromatic range. Using this system, we show that dorsal horn astrocytes in behaving mice show sensorimotor program-dependent and lamina-specific calcium excitation. Additionally, we show that tachykinin precursor 1 (Tac1)-expressing neurons exhibit translaminar activity to acute mechanical pain but not locomotion.

Date: 2023
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DOI: 10.1038/s41467-023-36959-2

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