An integrated single cell and spatial transcriptomic map of human white adipose tissue

Lucas Massier, Jutta Jalkanen, Merve Elmastas, Jiawei Zhong, Tongtong Wang, Pamela A. Nono Nankam, Scott Frendo-Cumbo, Jesper Bäckdahl, Narmadha Subramanian, Takuya Sekine, Alastair G. Kerr, Ben T. P. Tseng, Jurga Laurencikiene, Marcus Buggert, Magda Lourda, Karolina Kublickiene, Nayanika Bhalla, Alma Andersson, Armand Valsesia, Arne Astrup, Ellen E. Blaak, Patrik L. Ståhl, Nathalie Viguerie, Dominique Langin, Christian Wolfrum, Matthias Blüher, Mikael Rydén () and Niklas Mejhert ()
Additional contact information
Lucas Massier: Karolinska University Hospital Huddinge
Jutta Jalkanen: Karolinska University Hospital Huddinge
Merve Elmastas: Karolinska University Hospital Huddinge
Jiawei Zhong: Karolinska University Hospital Huddinge
Tongtong Wang: ETH Zurich
Pamela A. Nono Nankam: Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig
Scott Frendo-Cumbo: Karolinska University Hospital Huddinge
Jesper Bäckdahl: Karolinska University Hospital Huddinge
Narmadha Subramanian: Karolinska University Hospital Huddinge
Takuya Sekine: Karolinska Institutet, Karolinska University Hospital Huddinge
Alastair G. Kerr: Karolinska University Hospital Huddinge
Ben T. P. Tseng: Karolinska University Hospital Huddinge
Jurga Laurencikiene: Karolinska University Hospital Huddinge
Marcus Buggert: Karolinska Institutet, Karolinska University Hospital Huddinge
Magda Lourda: Karolinska Institutet, Karolinska University Hospital Huddinge
Karolina Kublickiene: Unit of Renal Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge
Nayanika Bhalla: KTH Royal Institute of Technology
Alma Andersson: KTH Royal Institute of Technology
Armand Valsesia: Nestle Institute of Health Sciences, Nestle Research
Arne Astrup: The Novo Nordisk Foundation
Ellen E. Blaak: Maastricht University Medical Centre(+)
Patrik L. Ståhl: KTH Royal Institute of Technology
Nathalie Viguerie: Université Toulouse III - Paul Sabatier (UPS), Université de Toulouse
Dominique Langin: Université Toulouse III - Paul Sabatier (UPS), Université de Toulouse
Christian Wolfrum: ETH Zurich
Matthias Blüher: Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig
Mikael Rydén: Karolinska University Hospital Huddinge
Niklas Mejhert: Karolinska University Hospital Huddinge

Nature Communications, 2023, vol. 14, issue 1, 1-19

Abstract: Abstract To date, single-cell studies of human white adipose tissue (WAT) have been based on small cohort sizes and no cellular consensus nomenclature exists. Herein, we performed a comprehensive meta-analysis of publicly available and newly generated single-cell, single-nucleus, and spatial transcriptomic results from human subcutaneous, omental, and perivascular WAT. Our high-resolution map is built on data from ten studies and allowed us to robustly identify >60 subpopulations of adipocytes, fibroblast and adipogenic progenitors, vascular, and immune cells. Using these results, we deconvolved spatial and bulk transcriptomic data from nine additional cohorts to provide spatial and clinical dimensions to the map. This identified cell-cell interactions as well as relationships between specific cell subtypes and insulin resistance, dyslipidemia, adipocyte volume, and lipolysis upon long-term weight changes. Altogether, our meta-map provides a rich resource defining the cellular and microarchitectural landscape of human WAT and describes the associations between specific cell types and metabolic states.

Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36983-2

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DOI: 10.1038/s41467-023-36983-2

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