PCR-like performance of rapid test with permselective tunable nanotrap

Seong Jun Park, Seungmin Lee, Dongtak Lee, Na Eun Lee, Jeong Soo Park, Ji Hye Hong, Jae Won Jang, Hyunji Kim, Seokbeom Roh, Gyudo Lee, Dongho Lee, Sung-Yeon Cho, Chulmin Park, Dong-Gun Lee, Raeseok Lee, Dukhee Nho, Dae Sung Yoon (), Yong Kyoung Yoo () and Jeong Hoon Lee ()
Additional contact information
Seong Jun Park: Kwangwoon University
Seungmin Lee: Kwangwoon University
Dongtak Lee: Korea University
Na Eun Lee: Kwangwoon University
Jeong Soo Park: Kwangwoon University
Ji Hye Hong: Kwangwoon University
Jae Won Jang: Korea University
Hyunji Kim: Korea University
Seokbeom Roh: Korea University
Gyudo Lee: Korea University
Dongho Lee: CALTH Inc.
Sung-Yeon Cho: The Catholic University of Korea
Chulmin Park: The Catholic University of Korea
Dong-Gun Lee: The Catholic University of Korea
Raeseok Lee: The Catholic University of Korea
Dukhee Nho: The Catholic University of Korea
Dae Sung Yoon: Korea University
Yong Kyoung Yoo: Catholic Kwandong University
Jeong Hoon Lee: Kwangwoon University

Nature Communications, 2023, vol. 14, issue 1, 1-10

Abstract: Abstract Highly sensitive rapid testing for COVID-19 is essential for minimizing virus transmission, especially before the onset of symptoms and in asymptomatic cases. Here, we report bioengineered enrichment tools for lateral flow assays (LFAs) with enhanced sensitivity and specificity (BEETLES2), achieving enrichment of SARS-CoV-2 viruses, nucleocapsid (N) proteins and immunoglobulin G (IgG) with 3-minute operation. The limit of detection is improved up to 20-fold. We apply this method to clinical samples, including 83% with either intermediate (35%) or low viral loads (48%), collected from 62 individuals (n = 42 for positive and n = 20 for healthy controls). We observe diagnostic sensitivity, specificity, and accuracy of 88.1%, 100%, and 91.9%, respectively, compared with commercial LFAs alone achieving 14.29%, 100%, and 41.94%, respectively. BEETLES2, with permselectivity and tunability, can enrich the SARS-CoV-2 virus, N proteins, and IgG in the nasopharyngeal/oropharyngeal swab, saliva, and blood serum, enabling reliable and sensitive point-of-care testing, facilitating fast early diagnosis.

Date: 2023
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DOI: 10.1038/s41467-023-37018-6

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