Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition

Zaida Vergara, María S. Gomez, Bénédicte Desvoyes, Joana Sequeira-Mendes, Kinda Masoud, Celina Costas, Sandra Noir, Elena Caro, Victoria Mora-Gil, Pascal Genschik and Crisanto Gutierrez ()
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Zaida Vergara: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
María S. Gomez: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
Bénédicte Desvoyes: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
Joana Sequeira-Mendes: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
Kinda Masoud: Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg
Celina Costas: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
Sandra Noir: Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg
Elena Caro: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
Victoria Mora-Gil: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco
Pascal Genschik: Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg
Crisanto Gutierrez: Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Nicolás Cabrera 1, Cantoblanco

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Most cellular proteins involved in genome replication are conserved in all eukaryotic lineages including yeast, plants and animals. However, the mechanisms controlling their availability during the cell cycle are less well defined. Here we show that the Arabidopsis genome encodes for two ORC1 proteins highly similar in amino acid sequence and that have partially overlapping expression domains but with distinct functions. The ancestral ORC1b gene, present before the partial duplication of the Arabidopsis genome, has retained the canonical function in DNA replication. ORC1b is expressed in both proliferating and endoreplicating cells, accumulates during G1 and is rapidly degraded upon S-phase entry through the ubiquitin-proteasome pathway. In contrast, the duplicated ORC1a gene has acquired a specialized function in heterochromatin biology. ORC1a is required for efficient deposition of the heterochromatic H3K27me1 mark by the ATXR5/6 histone methyltransferases. The distinct roles of the two ORC1 proteins may be a feature common to other organisms with duplicated ORC1 genes and a major difference with animal cells.

Date: 2023
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DOI: 10.1038/s41467-023-37024-8

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