Affinity maturation generates pathogenic antibodies with dual reactivity to DNase1L3 and dsDNA in systemic lupus erythematosus

Eduardo Gomez-Bañuelos, Yikai Yu, Jessica Li, Kevin S. Cashman, Merlin Paz, Maria Isabel Trejo-Zambrano, Regina Bugrovsky, Youliang Wang, Asiya Seema Chida, Cheryl A. Sherman-Baust, Dylan P. Ferris, Daniel W. Goldman, Erika Darrah, Michelle Petri, Iñaki Sanz and Felipe Andrade ()
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Eduardo Gomez-Bañuelos: The Johns Hopkins University School of Medicine
Yikai Yu: Huazhong University of Science and Technology
Jessica Li: The Johns Hopkins University School of Medicine
Kevin S. Cashman: Emory University
Merlin Paz: The Johns Hopkins University School of Medicine
Maria Isabel Trejo-Zambrano: The Johns Hopkins University School of Medicine
Regina Bugrovsky: Emory University
Youliang Wang: Emory University
Asiya Seema Chida: Emory University
Cheryl A. Sherman-Baust: National Institute on Aging
Dylan P. Ferris: The Johns Hopkins University School of Medicine
Daniel W. Goldman: The Johns Hopkins University School of Medicine
Erika Darrah: The Johns Hopkins University School of Medicine
Michelle Petri: The Johns Hopkins University School of Medicine
Iñaki Sanz: Emory University
Felipe Andrade: The Johns Hopkins University School of Medicine

Nature Communications, 2023, vol. 14, issue 1, 1-14

Abstract: Abstract Anti-dsDNA antibodies are pathogenically heterogeneous, implying distinct origins and antigenic properties. Unexpectedly, during the clinical and molecular characterization of autoantibodies to the endonuclease DNase1L3 in patients with systemic lupus erythematosus (SLE), we identified a subset of neutralizing anti-DNase1L3 antibodies previously catalogued as anti-dsDNA. Based on their variable heavy-chain (VH) gene usage, these antibodies can be divided in two groups. One group is encoded by the inherently autoreactive VH4-34 gene segment, derives from anti-DNase1L3 germline-encoded precursors, and gains cross-reactivity to dsDNA – and some additionally to cardiolipin – following somatic hypermutation. The second group, originally defined as nephritogenic anti-dsDNA antibodies, is encoded by diverse VH gene segments. Although affinity maturation results in dual reactivity to DNase1L3 and dsDNA, their binding efficiencies favor DNase1L3 as the primary antigen. Clinical, transcriptional and monoclonal antibody data support that cross-reactive anti-DNase1L3/dsDNA antibodies are more pathogenic than single reactive anti-dsDNA antibodies. These findings point to DNase1L3 as the primary target of a subset of antibodies classified as anti-dsDNA, shedding light on the origin and pathogenic heterogeneity of antibodies reactive to dsDNA in SLE.

Date: 2023
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DOI: 10.1038/s41467-023-37083-x

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