Turning universal O into rare Bombay type blood

Itxaso Anso, Andreas Naegeli, Javier O. Cifuente, Ane Orrantia, Erica Andersson, Olatz Zenarruzabeitia, Alicia Moraleda-Montoya, Mikel García-Alija, Francisco Corzana, Rafael A. Orbe, Francisco Borrego, Beatriz Trastoy (), Jonathan Sjögren () and Marcelo E. Guerin ()
Additional contact information
Itxaso Anso: Cruces University Hospital
Andreas Naegeli: Genovis AB
Javier O. Cifuente: Cruces University Hospital
Ane Orrantia: Cruces University Hospital
Erica Andersson: Genovis AB
Olatz Zenarruzabeitia: Cruces University Hospital
Alicia Moraleda-Montoya: Cruces University Hospital
Mikel García-Alija: Cruces University Hospital
Francisco Corzana: Universidad de La Rioja
Rafael A. Orbe: Biocruces Bizkaia Health Research Institute
Francisco Borrego: Cruces University Hospital
Beatriz Trastoy: Cruces University Hospital
Jonathan Sjögren: Genovis AB
Marcelo E. Guerin: Cruces University Hospital

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Red blood cell antigens play critical roles in blood transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency in H antigen, the Oh Bombay phenotype, can only be transfused with group Oh blood to avoid serious transfusion reactions. We discover FucOB from the mucin-degrading bacteria Akkermansia muciniphila as an α-1,2-fucosidase able to hydrolyze Type I, Type II, Type III and Type V H antigens to obtain the afucosylated Bombay phenotype in vitro. X-ray crystal structures of FucOB show a three-domain architecture, including a GH95 glycoside hydrolase. The structural data together with site-directed mutagenesis, enzymatic activity and computational methods provide molecular insights into substrate specificity and catalysis. Furthermore, using agglutination tests and flow cytometry-based techniques, we demonstrate the ability of FucOB to convert universal O type into rare Bombay type blood, providing exciting possibilities to facilitate transfusion in recipients/patients with Bombay phenotype.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-023-37324-z Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37324-z

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-37324-z

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().