Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product

P. Konstantin Richter, Paula Blázquez-Sánchez, Ziyue Zhao, Felipe Engelberger, Christian Wiebeler, Georg Künze, Ronny Frank, Dana Krinke, Emanuele Frezzotti, Yuliia Lihanova, Patricia Falkenstein, Jörg Matysik, Wolfgang Zimmermann (), Norbert Sträter () and Christian Sonnendecker ()
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P. Konstantin Richter: Leipzig University
Paula Blázquez-Sánchez: Leipzig University
Ziyue Zhao: Leipzig University
Felipe Engelberger: Leipzig University Medical School
Christian Wiebeler: Leipzig University
Georg Künze: Leipzig University Medical School
Ronny Frank: Leipzig University
Dana Krinke: Leipzig University
Emanuele Frezzotti: University of Parma
Yuliia Lihanova: Leipzig University
Patricia Falkenstein: Leipzig University
Jörg Matysik: Leipzig University
Wolfgang Zimmermann: Leipzig University
Norbert Sträter: Leipzig University
Christian Sonnendecker: Leipzig University

Nature Communications, 2023, vol. 14, issue 1, 1-11

Abstract: Abstract The recently discovered metagenomic-derived polyester hydrolase PHL7 is able to efficiently degrade amorphous polyethylene terephthalate (PET) in post-consumer plastic waste. We present the cocrystal structure of this hydrolase with its hydrolysis product terephthalic acid and elucidate the influence of 17 single mutations on the PET-hydrolytic activity and thermal stability of PHL7. The substrate-binding mode of terephthalic acid is similar to that of the thermophilic polyester hydrolase LCC and deviates from the mesophilic IsPETase. The subsite I modifications L93F and Q95Y, derived from LCC, increased the thermal stability, while exchange of H185S, derived from IsPETase, reduced the stability of PHL7. The subsite II residue H130 is suggested to represent an adaptation for high thermal stability, whereas L210 emerged as the main contributor to the observed high PET-hydrolytic activity. Variant L210T showed significantly higher activity, achieving a degradation rate of 20 µm h−1 with amorphous PET films.

Date: 2023
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DOI: 10.1038/s41467-023-37415-x

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