Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins

Chanhee Kim, Yongmin Kwon, Jaehoon Jeong, Minji Kang, Ga Seul Lee, Jeong Hee Moon, Hyo-Jun Lee, Youn-Il Park and Giltsu Choi ()
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Chanhee Kim: Korea Advanced Institute of Science and Technology
Yongmin Kwon: Korea Advanced Institute of Science and Technology
Jaehoon Jeong: Korea Advanced Institute of Science and Technology
Minji Kang: Korea Advanced Institute of Science and Technology
Ga Seul Lee: Korea Research Institute of Bioscience and Biotechnology
Jeong Hee Moon: Korea Research Institute of Bioscience and Biotechnology
Hyo-Jun Lee: Korea Research Institute of Bioscience and Biotechnology
Youn-Il Park: Chungnam National University
Giltsu Choi: Korea Advanced Institute of Science and Technology

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Phytochrome B (phyB) is a plant photoreceptor that forms a membraneless organelle called a photobody. However, its constituents are not fully known. Here, we isolated phyB photobodies from Arabidopsis leaves using fluorescence-activated particle sorting and analyzed their components. We found that a photobody comprises ~1,500 phyB dimers along with other proteins that could be classified into two groups: The first includes proteins that directly interact with phyB and localize to the photobody when expressed in protoplasts, while the second includes proteins that interact with the first group proteins and require co-expression of a first-group protein to localize to the photobody. As an example of the second group, TOPLESS interacts with PHOTOPERIODIC CONTROL OF HYPOCOTYL 1 (PCH1) and localizes to the photobody when co-expressed with PCH1. Together, our results support that phyB photobodies include not only phyB and its primary interacting proteins but also its secondary interacting proteins.

Date: 2023
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DOI: 10.1038/s41467-023-37421-z

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