The Trypanosoma cruzi Antigen and Epitope Atlas: antibody specificities in Chagas disease patients across the Americas

Alejandro D. Ricci, Leonel Bracco, Emir Salas-Sarduy, Janine M. Ramsey, Melissa S. Nolan, M. Katie Lynn, Jaime Altcheh, Griselda E. Ballering, Faustino Torrico, Norival Kesper, Juan C. Villar, Iván S. Marcipar, Jorge D. Marco and Fernán Agüero ()
Additional contact information
Alejandro D. Ricci: Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Leonel Bracco: Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Emir Salas-Sarduy: Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Janine M. Ramsey: Instituto Nacional de Salud Pública
Melissa S. Nolan: University of South Carolina
M. Katie Lynn: University of South Carolina
Jaime Altcheh: Ciudad Autónoma de Buenos Aires
Griselda E. Ballering: Ciudad Autónoma de Buenos Aires
Faustino Torrico: Fundación CEADES
Norival Kesper: Universidade de São Paulo
Juan C. Villar: Universidad Autónoma de Bucaramanga y Fundación Cardioinfantil — Instituto de Cardiología
Iván S. Marcipar: Universidad Nacional del Litoral
Jorge D. Marco: Universidad Nacional de Salta — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Fernán Agüero: Instituto de Investigaciones Biotecnológicas (IIB) — Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

Nature Communications, 2023, vol. 14, issue 1, 1-18

Abstract: Abstract During an infection the immune system produces pathogen-specific antibodies. These antibody repertoires become specific to the history of infections and represent a rich source of diagnostic markers. However, the specificities of these antibodies are mostly unknown. Here, using high-density peptide arrays we examined the human antibody repertoires of Chagas disease patients. Chagas disease is a neglected disease caused by Trypanosoma cruzi, a protozoan parasite that evades immune mediated elimination and mounts long-lasting chronic infections. We describe a proteome-wide search for antigens, characterised their linear epitopes, and show their reactivity on 71 individuals from diverse human populations. Using single-residue mutagenesis we revealed the core functional residues for 232 of these epitopes. Finally, we show the diagnostic performance of identified antigens on challenging samples. These datasets enable the study of the Chagas antibody repertoire at an unprecedented depth and granularity, while also providing a rich source of serological biomarkers.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-023-37522-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37522-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-37522-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().