The m6A reader YTHDC1 and the RNA helicase DDX5 control the production of rhabdomyosarcoma-enriched circRNAs

Dario Dattilo, Gaia Di Timoteo, Adriano Setti, Andrea Giuliani, Giovanna Peruzzi, Manuel Beltran Nebot, Alvaro Centrón-Broco, Davide Mariani, Chiara Mozzetta and Irene Bozzoni ()
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Dario Dattilo: Sapienza University of Rome
Gaia Di Timoteo: Sapienza University of Rome
Adriano Setti: Sapienza University of Rome
Andrea Giuliani: Sapienza University of Rome
Giovanna Peruzzi: Center for Life Nano- & Neuro-Science@Sapienza, Fondazione Istituto Italiano di Tecnologia (IIT)
Manuel Beltran Nebot: Sapienza University of Rome
Alvaro Centrón-Broco: Sapienza University of Rome
Davide Mariani: Center for Human Technologies@Istituto Italiano di Tecnologia (IIT)
Chiara Mozzetta: Institute of Molecular Biology and Pathology (IBPM), National Research Council (CNR) of Italy
Irene Bozzoni: Sapienza University of Rome

Nature Communications, 2023, vol. 14, issue 1, 1-15

Abstract: Abstract N6-Methyladenosine (m6A) is well-known for controlling different processes of linear RNA metabolism. Conversely, its role in the biogenesis and function of circular RNAs (circRNAs) is still poorly understood. Here, we characterize circRNA expression in the pathological context of rhabdomyosarcoma (RMS), observing a global increase when compared to wild-type myoblasts. For a set of circRNAs, such an increase is due to the raised expression of the m6A machinery, which we also find to control the proliferation activity of RMS cells. Furthermore, we identify the RNA helicase DDX5 as a mediator of the back-splicing reaction and as a co-factor of the m6A regulatory network. DDX5 and the m6A reader YTHDC1 are shown to interact and to promote the production of a common subset of circRNAs in RMS. In line with the observation that YTHDC1/DDX5 depletion reduces RMS proliferation, our results provide proteins and RNA candidates for the study of rhabdomyosarcoma tumorigenicity.

Date: 2023
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DOI: 10.1038/s41467-023-37578-7

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