Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction

Miaowei Mao, Yajie Qian, Wenyao Zhang, Siyu Zhou, Zefeng Wang, Xianjun Chen () and Yi Yang ()
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Miaowei Mao: East China University of Science and Technology
Yajie Qian: East China University of Science and Technology
Wenyao Zhang: East China University of Science and Technology
Siyu Zhou: East China University of Science and Technology
Zefeng Wang: University of Chinese Academy of Sciences, Chinese Academy of Sciences
Xianjun Chen: East China University of Science and Technology
Yi Yang: East China University of Science and Technology

Nature Communications, 2023, vol. 14, issue 1, 1-13

Abstract: Abstract Optogenetics tools for precise temporal and spatial control of protein abundance are valuable in studying diverse complex biological processes. In the present study, we engineer a monomeric tag of stabilization upon light induction (SULI) for yeast and zebrafish based on a single light-oxygen-voltage domain from Neurospora crassa. Proteins of interest fused with SULI are stable upon light illumination but are readily degraded after transfer to dark conditions. SULI shows a high dynamic range and a high tolerance to fusion at different positions of the target protein. Further studies reveal that SULI-mediated degradation occurs through a lysine ubiquitination-independent proteasome pathway. We demonstrate the usefulness of SULI in controlling the cell cycle in yeast and regulating protein stability in zebrafish, respectively. Overall, our data indicate that SULI is a simple and robust tool to quantitatively and spatiotemporally modulate protein levels for biotechnological or biomedical applications.

Date: 2023
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DOI: 10.1038/s41467-023-37830-0

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