RPL3L-containing ribosomes determine translation elongation dynamics required for cardiac function

Chisa Shiraishi, Akinobu Matsumoto (), Kazuya Ichihara, Taishi Yamamoto, Takeshi Yokoyama, Taisuke Mizoo, Atsushi Hatano, Masaki Matsumoto, Yoshikazu Tanaka, Eriko Matsuura-Suzuki, Shintaro Iwasaki, Shouji Matsushima, Hiroyuki Tsutsui and Keiichi I. Nakayama ()
Additional contact information
Chisa Shiraishi: Kyushu University
Akinobu Matsumoto: Kyushu University
Kazuya Ichihara: Kyushu University
Taishi Yamamoto: Kyushu University
Takeshi Yokoyama: Tohoku University
Taisuke Mizoo: Kyushu University
Atsushi Hatano: Niigata University
Masaki Matsumoto: Niigata University
Yoshikazu Tanaka: Tohoku University
Eriko Matsuura-Suzuki: RIKEN Cluster for Pioneering Research
Shintaro Iwasaki: RIKEN Cluster for Pioneering Research
Shouji Matsushima: Kyushu University
Hiroyuki Tsutsui: Kyushu University
Keiichi I. Nakayama: Kyushu University

Nature Communications, 2023, vol. 14, issue 1, 1-17

Abstract: Abstract Although several ribosomal protein paralogs are expressed in a tissue-specific manner, how these proteins affect translation and why they are required only in certain tissues have remained unclear. Here we show that RPL3L, a paralog of RPL3 specifically expressed in heart and skeletal muscle, influences translation elongation dynamics. Deficiency of RPL3L-containing ribosomes in RPL3L knockout male mice resulted in impaired cardiac contractility. Ribosome occupancy at mRNA codons was found to be altered in the RPL3L-deficient heart, and the changes were negatively correlated with those observed in myoblasts overexpressing RPL3L. RPL3L-containing ribosomes were less prone to collisions compared with RPL3-containing canonical ribosomes. Although the loss of RPL3L-containing ribosomes altered translation elongation dynamics for the entire transcriptome, its effects were most pronounced for transcripts related to cardiac muscle contraction and dilated cardiomyopathy, with the abundance of the encoded proteins being correspondingly decreased. Our results provide further insight into the mechanisms and physiological relevance of tissue-specific translational regulation.

Date: 2023
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DOI: 10.1038/s41467-023-37838-6

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