Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins

Shintaroh Kubo, Corbin S. Black, Ewa Joachimiak, Shun Kai Yang, Thibault Legal, Katya Peri, Ahmad Abdelzaher Zaki Khalifa, Avrin Ghanaeian, Caitlyn L. McCafferty, Melissa Valente-Paterno, Chelsea Bellis, Phuong M. Huynh, Zhe Fan, Edward M. Marcotte, Dorota Wloga () and Khanh Huy Bui ()
Additional contact information
Shintaroh Kubo: McGill University
Corbin S. Black: McGill University
Ewa Joachimiak: Nencki Institute of Experimental Biology of Polish Academy of Sciences
Shun Kai Yang: McGill University
Thibault Legal: McGill University
Katya Peri: McGill University
Ahmad Abdelzaher Zaki Khalifa: McGill University
Avrin Ghanaeian: McGill University
Caitlyn L. McCafferty: University of Texas
Melissa Valente-Paterno: McGill University
Chelsea Bellis: McGill University
Phuong M. Huynh: McGill University
Zhe Fan: McGill University
Edward M. Marcotte: University of Texas
Dorota Wloga: Nencki Institute of Experimental Biology of Polish Academy of Sciences
Khanh Huy Bui: McGill University

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.

Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-023-37868-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37868-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-023-37868-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().