Lysosomal-associated protein transmembrane 5 ameliorates non-alcoholic steatohepatitis by promoting the degradation of CDC42 in mice

Lang Jiang, Jing Zhao, Qin Yang, Mei Li, Hao Liu, Xiaoyue Xiao, Song Tian, Sha Hu, Zhen Liu, Peiwen Yang, Manhua Chen (), Ping Ye () and Jiahong Xia ()
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Lang Jiang: Huazhong University of Science and Technology
Jing Zhao: Huazhong University of Science and Technology
Qin Yang: Huanggang Central Hospital
Mei Li: Wuhan University
Hao Liu: Huazhong University of Science and Technology
Xiaoyue Xiao: Huazhong University of Science and Technology
Song Tian: Wuhan University
Sha Hu: Wuhan University
Zhen Liu: Renmin Hospital of Wuhan University
Peiwen Yang: Huazhong University of Science and Technology
Manhua Chen: Huazhong University of Science and Technology
Ping Ye: Huazhong University of Science and Technology
Jiahong Xia: Huazhong University of Science and Technology

Nature Communications, 2023, vol. 14, issue 1, 1-16

Abstract: Abstract Non-alcoholic steatohepatitis (NASH) has received great attention due to its high incidence. Here, we show that lysosomal-associated protein transmembrane 5 (LAPTM5) is associated with NASH progression through extensive bioinformatical analysis. The protein level of LAPTM5 bears a negative correlation with NAS score. Moreover, LAPTM5 degradation is mediated through its ubiquitination modification by the E3 ubquitin ligase NEDD4L. Discovered by experiments conducted on male mice, hepatocyte-specific depletion of Laptm5 exacerbates mouse NASH symptoms. In contrast, Laptm5 overexpression in hepatocytes exerts diametrically opposite effects. Mechanistically, LAPTM5 interacts with CDC42 and promotes its degradation through a lysosome-dependent manner under the stimulation of palmitic acid, thus inhibiting activation of the mitogen-activated protein kinase signaling pathway. Finally, adenovirus-mediated hepatic Laptm5 overexpression ameliorates aforementioned symptoms in NASH models.

Date: 2023
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DOI: 10.1038/s41467-023-37908-9

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